BiTTE® - Microbial Analysis

Sample

Urine Specimen

The Primary category classifies results into five basic morphologies, "No bacteria" and "Multiple bacteria". Yeast refers to yeast-like fungi. These five basic morphologies are further subdivided into nine categories in the Secondary level and 15 species-level categories in the Tertiary level. It should be noted that "Multiple bacteria" always refers to a combination of the five basic morphologies, regardless of the category level, and does not include combinations of the subdivided categories.

Primary category	Secondary category	Tertiary category
Yeast	Yeast	Candida spp.
GPR	GPR	Corynebacterium spp.
GPC	GPC-cluster	GPC cluster
		GPC-chain
		Enterococcus faecium
		Streptococcus agalactiae
	GPC-other	Other GPC
GNR	GNR-L-M	Enterobacter cloacae
	GNR-L	Escherichia coli
		Klebsiella oxytoca
		Klebsiella pneumoniae
	GNR-S	Pseudomonas aeruginosa
		Other GNR Enterobacteriaceae
		Other GNR Glucose non-fermenting bacteria
GNC	GNC	GNC
No bacteria
Multiple bacteria

Blood Specimen Aerobic culture

The Primary category classifies results into five basic morphologies, "No bacteria" and "Multiple bacteria". Yeast refers to yeast-like fungi. These five basic morphologies are further subdivided into ten categories in the Secondary level and 19 species-level categories in the Tertiary level. It should be noted that "Multiple bacteria" always refers to a combination of the five basic morphologies, regardless of the category level, and does not include combinations of the subdivided categories.

Primary category	Secondary category	Tertiary category
Yeast	Yeast	Candida spp.
GPR	GPR-O	Listeria monocytogenes
		Other GPR
	GPR-C	Corynebacterium spp.
GPC	GPC-C	Staphylococcus aureus (MRSA/MSSA)
		Staphylococci (excl. S.aureus)
	GPC-E	Enterococcus faecium
		Enterococcus spp. (excl. E. faecium)
	GPC-S	β-Streptococcus (incl. S. pneumoniae)

Blood Specimen Anaerobic culture

The Primary category classifies results into three basic morphologies (GPR, GPC, GNR), "No bacteria" and "Multiple bacteria". These five basic morphologies are further subdivided into ten categories in the Secondary level and 14 species-level categories in the Tertiary level. It should be noted that "Multiple bacteria" always refers to a combination of the three basic morphologies, regardless of the category level, and does not include combinations of the subdivided categories.

Primary category	Secondary category	Tertiary category
GPR	GPR-O	Listeria monocytogenes
		Other GPR
	GPR-C	Corynebacterium spp.
GPC	GPC-C	Staphylococcus aureus (MRSA/MSSA)
		Staphylococci (excl. S.aureus)
	GPC-E	Enterococcus faecium
		Enterococcus spp. (excl. E. faecium)
	GPC-S	β-Streptococcus (incl. S. pneumoniae)
		Streptococci (excl. β-Streptococcus)

Key Features

1. Analyze Gram stain images to automatically estimate bacterial morphology using deep learning technology.

2. Classify microorganisms accurately into five basic morphologies, "Multiple bacteria," or "No bacteria."

3. Enables advanced classification into detailed subcategories beyond the five primary morphologies.

4. Analyze a Gram stain image and display the result in just a few seconds.

5. Results are automatically saved to a gallery for easy access.

6. Seamlessly share and discuss results with your team, just like any standard image.

Benefits

• Consistent Accuracy: Independent of the observer.
• Enhanced Efficiency: Through rapid analysis.
• Seamless Data Tracking: With automatic archiving.
• Collaborative Insights: Streamlined cross-checking.

How It Works

1. Capture an image of a Gram-stained slide at 1000x magnification using a smartphone or PC camera, then upload it to CarbConnect.

2. Select the captured image and choose the "BiTTE - Microbial Analysis" app from the sidebar on the left side of the screen.

3. Press the "Analyze" button to start the process. The AI will classify the bacterial morphology based on the uploaded image.

4. Verify the estimated results. You can modify the results or manually add specific species information as needed.

5. Save: Both the initial and adjusted results will be saved as images in the gallery.

Caution When Make Specimen

• Please perform Gram staining using the Bartholomew method.
• Ensure that the specimens used are free of staining defects.

Caution When Photographing

• Please do not use images that are out of focus.

Acknowledgement

This product has been developed in collaboration with the School of Medicine, Kobe University, the National Center for Global Health and Medicine (NCGM), and the Japan Agency for Medical Research and Development (AMED).

Other remarks

• For the smartphone version, please refer to BiTTE - iE.
• For guidance on achieving high-quality Gram staining, please refer to the automated gram stainer - Point of Care Gram Stainer (“PoCGS”).